The present study was conducted to investigate the bacterial infections of urinary tract and their antibiotic resistance pattern in Dhaka city in the Bangladesh. The study was carried out from November 2016 until July 2017. Two hundred and thirty-five patients with urolithiasis were included in the present study whose ages ranged from 05 to 70 years. Seven different bacterial species with noticeable growth were found in the urine sample after bacterial cultures were performed. E. coli was the most frequent bacterium found in patients’ urinary tract infections, accounting for 50% of cases. Klebsiella (12%), Pseudomonas (10%), Proteus (10%), Streptococcus (8%), Citobacter (6%) and Staphylococcus (4%), were the next most frequent bacteria. According to E. coli, women (72%) had more urinary tract infections than men (21.28%). Diverse bacterial pathogens were discovered to be extremely susceptible to cotrimoxazole, ciprofloxacin, and the most of them were also quite sensitive to nitrofurantoin and nalidixic acid. Furthermore, nalidixic acid, cotrimoxazole, nitrofurantoin, and imipenem showed considerable sensitivity to E. coli, Klebsiella spp., Pseudomonas spp., Proteus spp., Staphylococcus spp., Streptococcus spp., and Citrobacter spp.